anti ngfr  (Cell Signaling Technology Inc)

Journal: bioRxiv

Article Title: AID Shapes Proliferation and Cell-of-Origin-associated Transcriptional Programs in Diffuse Large B-cell Lymphoma

doi: 10.64898/2026.02.24.707639

Figure Lengend Snippet: A-B , Immunoblot analysis showing AID-ERα expression in three independent AID knockout clones of HBL1 ( A ) and TMD8 ( B ) cells transduced with either the AID-Erα fusion construct or the ΔNGFR control construct. β-actin was used as a loading control. C , GSEA plots depicting enrichment of MYC and E2F target gene sets in pooled independent TMD8 and HBL1 AID-ERα and ΔNGFR clones. FDR and NES values are shown in the plots. D , Bar graph showing enriched pathways identified by pooled gene set enrichment analysis in AID-ERα and ΔNGFR cell clones. Gene sets with a false discovery rate (FDR) < 0.2 are displayed, with AID-ERα–enriched gene sets shown in red and ΔNGFR-enriched gene sets in blue. The x-axis indicates enrichment scores. E-F , Venn diagrams illustrating the overlap of leading-edge (LE) genes from the MYC and E2F target gene sets across HBL1 and TMD8 clones overexpressing AID-ERα.

Article Snippet: Following transduction, cells were expanded and sorted for ΔNGFR expression by FACS, selecting the top 10% of ΔNGFR-positive cells using anti-NGFR antibody (ME20.4-1.H4, Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Western Blot, Expressing, Knock-Out, Clone Assay, Transduction, Construct, Control

Journal: bioRxiv

Article Title: AID Shapes Proliferation and Cell-of-Origin-associated Transcriptional Programs in Diffuse Large B-cell Lymphoma

doi: 10.64898/2026.02.24.707639

Figure Lengend Snippet: A , GSEA of ABC (left panels) and GCB (right panels) stratification genes in biological triplicates of GCB-type DLBCL cell lines SU-DHL-6 (upper panels) and OCI-LY18 (lower panels) expressing AID-ERα or ΔNGFR control. FDR and NES values are indicated in the enrichment plots. B , Heatmap showing the z-score-normalized expression of ABC and GCB stratification genes in SU-DHL-6 cells. C , Venn diagram of ABC stratification genes enriched in AID-ERα expressing GCB-type DLBCL cell lines SU-DHL-6 and OCI-LY18 (left panel). Venn diagram of GCB stratification genes enriched in ΔNGFR control cell lines (right panel).

Article Snippet: Following transduction, cells were expanded and sorted for ΔNGFR expression by FACS, selecting the top 10% of ΔNGFR-positive cells using anti-NGFR antibody (ME20.4-1.H4, Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Expressing, Control

Journal: bioRxiv

Article Title: AID Shapes Proliferation and Cell-of-Origin-associated Transcriptional Programs in Diffuse Large B-cell Lymphoma

doi: 10.64898/2026.02.24.707639

Figure Lengend Snippet: A–D , Immunoblot analysis of AID-ERα ( A ), p105, p50 ( B ), BCL6, IRF4 ( C ) and MYC ( D ) protein expression in biological triplicates of the GCB-type DLBCL cell line SU-DHL-6 overexpressing AID-ERα or ΔNGFR control cells. Cells were seeded and maintained at 0.3 × 10^6 cells/ml with passaging every three days for 11 days in the presence of tamoxifen. E–J , Quantification of protein expression by densitometry of AID-ERα ( E ), p105 ( F ), p50 ( G ), BCL6 ( H ), IRF4 ( I ) and MYC ( J ). Statistical significance was assessed using a two-tailed unpaired t -test (* p<0.05; ** p<0.01; **** p<0.0001). K , Enrichment plots of ABC (first and third panels) and GCB (second and fourth panels) stratification gene sets from GSEA analyses of ABC-type (left two panels) and GCB-type (right two panels) DLBCL patients from Sha et al. Comparisons were performed between patients in the upper versus lower quartile of AID expression. L , Bar graphs showing the number of ABC and GCB stratification genes with higher expression in the upper quartile versus the lower quartile of AID expression (grey) or higher expressed in the lower quartile than the upper quartile of AID expression (black), stratified by ABC-type and GCB-type DLBCL patients from Sha et al . M , Venn diagrams depicting overlap in enriched genes: ABC stratification genes enriched in the AID upper quartile of both ABC-type and GCB-type DLBCL (left panel), and GCB stratification genes enriched in the AID lower quartile of both ABC-type and GCB-type DLBCL (right panel).

Article Snippet: Following transduction, cells were expanded and sorted for ΔNGFR expression by FACS, selecting the top 10% of ΔNGFR-positive cells using anti-NGFR antibody (ME20.4-1.H4, Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Western Blot, Expressing, Control, Passaging, Two Tailed Test